How to Operate a Spectrophotometer

Spectrophotometric solutions simplify the science of quantifying chromatic data for many industries. Manufacturers using a spectrophotometer benefit from higher product quality and consistency, increasing customer satisfaction. Accurately quantifying color and appearance starts with learning how to use a spectrophotometer correctly and implementing a standardized process.

1. Turn the Device on and Allow It to Warm Up

The first step in preparing a spectrophotometer for use is to turn it on and allow it to warm up so it can function correctly. Warmup times can vary depending on the model and manufacturer, but you'll generally want to wait 30 minutes. To protect the integrity of your outcomes, verify the exact time frame by consulting your owner's manual.

2. Clean and Prepare Containers and Control Samples

We recommend gently cleaning with specialized glass wipes or deionized water and a soft, lint-free cloth to prevent container damage.

Next, prepare your control sample according to the instructions specified by the manufacturer. You should use the same type and size of container and the same solution you measure your actual product samples in.

3. Standardize the Device According to Manufacturer's Directions

Your spectrophotometer's user manual is the best source of information on how to standardize the device. Approaches will differ based on whether your color measurement workflows quantify transmittance or reflectance.

Transmittance can be measured on solutions like the HunterLab Vista as either Total Transmittance (TTRAN) or Regular Transmittance (RTRAN). This process uses blank cells and solutions, with sample positioning determining whether to choose TTRAN or RTRAN readouts — standardizing for haze requires the TTRAN mode.

The process differs slightly for spectrophotometers measuring reflectance, like the ColorFlex L2. You'll use the standardized black glass, white tile. and green diagnostic check tile. Start with the black, then the white, and finally the green tile. Acceptable measurement values for the green tile appear on its reverse so you can easily verify that the equipment is standardized correctly.s

4. Choose Your Measurement Comparisons and Capture Data

After standardization, you're ready to take measurements on your product samples. Choose the appropriate custom tolerances, scales, or indices that apply to quantify the data within a recognized standard. You'll repeat step two to clean test tubes or cuvettes before measuring. Add the sample to the sampling chamber, taking care to orient it correctly for the light source's direction. Record values in either reflection or transmittance percentages and compare them against your standards to assess conformance.

Invest in Accurate Color Science With HunterLab

HunterLab has been a pioneer in color science measurement since 1952. Our spectrophotometric solutions support operations in quality assurance labs worldwide with unmatched precision, accuracy, and ease of use.

Take advantage of our more than 70 years of expertise and contact our experts online to find the best device for your application and request a quote.